Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Stefan Robert Marsden, Hector Sanguesa Ferrer
Date created: 2015-09-07 11:00:00
Date modified: 2015-09-18 11:07:01
pRha + CsgA + Hydroxyapatite-affinity tag
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K1583102_sequence (Version 1) |
Description
This part was synthesized. The rhamnose promoter was used by the iGEM 2014 TU Delft team and originally added by the iGEM12 Paris Bettencourt team. RBS and CsgA originate from E. coli K-12 MG1655.The nucleotides encoding for the second and third amino acid of the CsgA gene were changed to optimize synthesis success. (silent mutations).
Upon induction with rhamnose, CsgA is expressed and exported into the extracellular space. There, the membrane protein CsgB can trigger the CsgA protein monomers to aggregate and form amyloid nanofibres.
Notes
The stop codon needed to be removed from CsgA to add the N-terminal hydroxyapatite affinity tag.Source
The DNA was synthesized. The sequence originates from the genomic DNA of E. coli K-12 MG1655 (http://www.ncbi.nlm.nih.gov/gene/949055).| Access | Instance | Definition |
| public public public public public public public | BBa_K914003 BBa_K1583050 BBa_K1583008 BBa_K1583014 BBa_K1583015 BBa_K1583060 BBa_K1583051 | BBa_K914003 BBa_K1583050 BBa_K1583008 BBa_K1583014 BBa_K1583015 BBa_K1583060 BBa_K1583051 |