Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Leung King Pong
Date created: 2015-07-16 11:00:00
Date modified: 2015-09-23 11:07:34
Insertion Kit for Fusing Protein of Interest to Magnetosome Membrane
| Types | DnaRegion |
| Roles | Plasmid_Backbone plasmid_vector |
| Sequences | BBa_K1648004_sequence (Version 1) |
Description
Consist of six fragments from magnetosome island of Magnetospirillum gryphiswaldense genome.
Due to potential difficulty in introducing the large magnetosome formation operons into Azotobacter vinelandii, a "template" consists of flanking sequences from mamXY, mamGFDC and mms6 operons except the mamC operon is designed for homologous recombination.
Part containing this template (K1648002) together with another part containing mamC (K1648XXX) is introduced into A. vinelandii genome via random integration before full operon sequences to be transformed into A. vinelandii.
Notes
In order to form a homologous recombination template, we selected 250 bp of 5' and 3' ends from mamGFDC, mamYXZ-ftsZ and mms6-mmsF-mms35-mms48 (mamGC, mamXY and mms6 operons) except the mamC operon as flanking sequences. Fragments are joined via overlapping PCR.
Source
Magnetospirillum gryphiswaldense MSR-1 v2, complete genome| Sequence Annotation | Location | Component / Role(s) |
| TetR 1 BBa_R0040 -35 TetR 2 -10 BBa_B0034 conserved BBa_J13002 mamC BBa_K1648007 BBa_B0010 stem_loop BBa_B0012 T7 TE polya stop BBa_B0015 | 1,19 1,54 20,25 26,44 43,48 63,74 67,70 1,74 81,455 81,455 464,543 475,518 552,592 559,578 579,592 585,585 464,592 | feature/binding non_covalent_binding_site feature/BioBrick engineered_region promoter feature/promoter non_covalent_binding_site feature/binding feature/promoter promoter engineered_region feature/BioBrick feature/conserved conserved_region feature/BioBrick engineered_region feature/misc sequence_feature feature/BioBrick engineered_region engineered_region feature/BioBrick feature/stem_loop stem_loop feature/BioBrick engineered_region feature/stem_loop stem_loop feature/polya polyA_site feature/stop stop_codon engineered_region feature/BioBrick |