Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Marta Żardecka
Date created: 2015-09-16 11:00:00
Date modified: 2015-09-17 07:06:25
sfGFP under proD promoter
| Types | DnaRegion |
| Roles | Generator engineered_region |
| Sequences | BBa_K1741014_sequence (Version 1) |
Description
A strong insulated constitutive promoter proD is stronger than proC. Expression profiles of proC and proD are similar on different media = both seem to be really constitutive and the expression level does depend on E. coli growth rate.Notes
None.Source
All promoter fragments derive from Escherichia coli K12, strain DH5α, some elements are synthetic. Chromosomal DNA of E. coli was isolated as described in the methods section.| Sequence Annotation | Location | Component / Role(s) |
| proD promoter -35 signal -10 signal 5'UTR His-tag sfGFP two consecutive STOP codons | 1,101 63,68 86,91 102,158 159,188 189,902 903,908 | feature/promoter promoter feature/misc sequence_feature sequence_feature feature/misc sequence_feature feature/misc tag feature/tag CDS feature/protein feature/stop stop_codon |