Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Madeleine Stone
Date created: 2016-10-11 11:00:00
Date modified: 2016-10-12 09:46:00
promoter + CBZ cleavage enzyme + terminator
| Types | DnaRegion |
| Roles | engineered_region Device |
| Sequences | BBa_K1879001_sequence (Version 1) |
Description
This part contains the CBZ-cleavage enzyme with a promoter (BBa_J23118) and RBS upstream of the coding sequence, and terminator (BBa_J61048) downstream of the sequence to make a fully functioning BioBrick. This BioBrick is used in our biocontainment system to cleave the CBZ protecting group from leucine prior to incorporation of leucine into proteins. This part can be used by other iGEM teams to selectively cleave the CBZ protecting group from amino or hydroxyl groups in organic reactions.Notes
The promoter and terminator were cloned into the vector containing the cleavage enzyme.Source
The coding sequence of this CBZ cleavage enzyme came from a study by Patel et al. See Part:BBa_K1879000, which is just the enzyme coding sequence. The promoter (BBa_J23118) and terminator (BBa_J61048) came from the iGEM kit.| Access | Instance | Definition |
| public public public | BBa_J23118 BBa_K1879000 BBa_J61048 | BBa_J23118 BBa_K1879000 BBa_J61048 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J23118 BBa_K1879000 BBa_J61048 | 1,35 44,1394 1403,1515 | BBa_J23118 BBa_K1879000 BBa_J61048 |