Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Bernardo Pollak, Isaac Nu??ez
Date created: 2012-09-19 11:00:00
Date modified: 2015-05-08 01:13:09
psb1C3_IntKR recombination plasmid for Synechocystis PCC6803
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K743008_sequence (Version 1) |
Description
This plasmid allows the integration of a double terminator followed by a reversed Kanamycin resistance casette in to Synechocystis chromosome, making possible selection in this cyanobacteria. It has a Chloramphenicol resistance casette for selection in E.coliThe use of BBa_K743000 and BBa_K743001 as recombination sites has no deleterious phenotypic effects on the cells.
The plasmid is intended to be used as a starting backbone for further adition of dna parts by Gibson assembly.
It was used by UC_Chile 2012 team to succesfully transform Synechocystis
Notes
Using a double terminator followed by a reversed antibiotic resistance cds allows the use of the same terminator sequence for both the resistance casette and any desired coding sequence placed upstreamSource
Both BBa_K743001 and BBa_K743002 were PCR amplified from genomic dna. BBa_K743001 and BBa_B0015 were amplified from biobricks.| Access | Instance | Definition |
| public public public | BBa_K743000 BBa_S05073 BBa_K743001 | RS1 BBa_S05073 RS2 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K743000 BBa_S05073 BBa_K743001 | 1,522 523,1626 1627,2313 | RS1 BBa_S05073 RS2 |