BBa_K912006

BBa_K912006 Version 1

Component

This part has been discontinued.

Please consider using BBa_S05032 as a replacement.

Source: http://parts.igem.org/Part:BBa_K912006

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Grant Nicholas

Date created: 2012-09-27 11:00:00

Date modified: 2015-05-08 01:13:45

Intracellular Chloride Ion Inducible Lysis Device

Details

• Types: DnaRegion
• Roles: Composite; engineered_region
• Sequences: BBa_K912006_sequence (Version 1)

Description

This construct was developed by Northwestern's 2012 iGEM team as part of a phytase producing vector meant to self lyse in high chloride and low pH conditions. The device is designed to lyse in the presence of high chloride concentrations. The Pgad promoter in front of the lysis cassette is activated by the combination of high extracellular hydrogen ion concentration and the presence of high intracellular chloride ion concentration.

Notes

Pgad did not come from the registry. The CUHK team sent the part in an unknown vector and the Pgad promoter was PCR'd out.

Source

Pgad: CUHK 2011 iGEM team
Lysis: parts registry

Components

Access	Instance	Definition
public	BBa_K559011	Pgad
public	BBa_K112808	BBa_K112808

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K559011	1,1251	Pgad
BBa_K112808	1260,3044	BBa_K112808

Other Properties

• igem#experience: None
• igem#sampleStatus: Discontinued
• igem#status: Deleted
• isReplacedBy: BBa_S05032/1
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K912006/1

Member of these Collections

• iGEM Parts Registry