Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Ashley Hammerbacher Celina Malav??
Date created: 2014-04-29 11:00:00
Date modified: 2015-05-08 01:14:03
Winter Flounder Antifreeze Protein Actuator With Secretion Tag
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_M36085_sequence (Version 1) |
Description
This is our actuator to generate antifreeze protein activity in yeast. We are expressing the winter flounder AFP to be able to minimize ice growth. It includes the yeast secretion tag so that it is expressed outside of the cell and so that it will be expressed in the solution.Notes
We had to make sure that we had everything that could express the protein in yeast (Kozac sequence, Yeast secretion Tag) and optimized our sequences for yeast.Source
The AFP comes from Uniprot and the parts come from the parts registry.| Access | Instance | Definition |
| public public public public public | BBa_K792001 BBa_M36082 BBa_K416003 BBa_M36084 BBa_K392003 | MFa1-Kozak BBa_M36082 BBa_K416003 BBa_M36084 BBa_K392003 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K792001 BBa_M36082 BBa_K416003 BBa_M36084 BBa_K392003 | 1,21 28,273 280,393 402,422 431,559 | MFa1-Kozak BBa_M36082 BBa_K416003 BBa_M36084 BBa_K392003 |