Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Zach Banks
Date created: 2012-05-14 11:00:00
Date modified: 2015-05-08 01:14:07
DNAseI generator inhibited by recombinant pCat promoter
| Types | DnaRegion |
| Roles | Device engineered_region |
| Sequences | BBa_M39412_sequence (Version 1) |
Description
DNAseI generating module that is inhibited by (lack of) recombination.Initially, the promoter is set to produce actin with a fast degradation tag, which inhibits DNAseI.
When fimE is expressed elsewhere in the cell, the promoter is flipped to stop production of actin. Instead, DNAseI is expressed, along with ECP32 which degrades actin. Additionally, tetR is produced to be used in other regulatory mechanisms.
Notes
There is a terminator before the pCat promoter to prevent "backwards" expression, which would be detrimental to the strong "digital" behavior the recombinant region attempts to achieve.- Everything before the fimE IRL should be on the antisense strand, and thus inverted.
| Access | Instance | Definition |
| public public public public public public public public public public public public | BBa_B0015 BBa_C0040 BBa_J61106 BBa_J61105 BBa_J61104 BBa_K137010 BBa_B0015 BBa_I14033 BBa_K137008 BBa_J61103 BBa_M0050 BBa_B0015 | BBa_B0015 tetR BBa_J61106 BBa_J61105 BBa_J61104 fimE IRL BBa_B0015 Cat IRR BBa_J61103 LAA BBa_B0015 |