BBa_K1583113

BBa_K1583113 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1583113

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Max van

Date created: 2015-09-13 11:00:00

Date modified: 2015-09-17 05:21:01

pAra + fusion protein BFP_Spycatcher_His

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_K1583113_sequence (Version 1)

Description

Inducible arabinose promoter controlling expression of BFP_SpyCatcher_His.

Notes

The start condon of SpyCatcher and the stop codon of BFP needed to be removed to ensure that one complete protein was expressed.

Source

These part was synthesized as linear DNA and only restriction/ligation was done to insert this into the backbone.

Components

Access	Instance	Definition
public	BBa_K1583054	BBa_K1583054
public	BBa_K206000	pBAD
public	BBa_G0002	SX scar
public	BBa_K1583061	BBa_K1583061
public	BBa_K1583017	BBa_K1583017
public	BBa_K1583018	BBa_K1583018

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K1583054	1,6	BBa_K1583054
BBa_K206000	7,136	pBAD
BBa_G0002	137,144	SX scar
BBa_K1583061	147,158	BBa_K1583061
BBa_K1583017	162,860	BBa_K1583017
BBa_K1583018	861,1268	BBa_K1583018

Other Properties

• igem#experience: Issues
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1583113/1

Member of these Collections

• iGEM 2018 Distribution Plate 7
• iGEM 2018 Distribution Plate 7 Well 5J
• iGEM 2018 Part Library
• iGEM 2018 Plasmid pSB1C3
• iGEM Parts Registry