BBa_K2004001
BBa_K2004001 Version 1 (Component)
Construct of CDO

BBa_K2004010
BBa_K2004010 Version 1 (Component)
CSAD Construct

BBa_M50033
BBa_M50033 Version 1 (Component)
U1_mTurquoise2, IPTG-induced, CAM resistant Thermosensor Construct

BBa_M50034
BBa_M50034 Version 1 (Component)
U10_mKO2, IPTG inducible, KAN resistant Thermosensor Construct

SBOLDesigner CAD Tool
SBOLDesigner Version 3.1 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.

BBa_K299816
BBa_K299816 Version 1 (Component)
control construct - invasin + GFP under J23102

BBa_K299817
BBa_K299817 Version 1 (Component)
control construct - GFP under J23102

BBa_K300085
BBa_K300085 Version 1 (Component)
BBa_K300086 construction intermediate

BBa_K300087
BBa_K300087 Version 1 (Component)
BBa_K300088 construction intermediate

BBa_K300089
BBa_K300089 Version 1 (Component)
BBa_K300090 construction intermediate

BBa_K322710
BBa_K322710 Version 1 (Component)
Marker construct which can be used to target tnaA in E.coli.

BBa_K392045
BBa_K392045 Version 1 (Component)
endoglucanase secretion testing construct for yeast

BBa_M1007
BBa_M1007 Version 1 (Component)
Complete Phasin Secretion Construct

BBa_M1115
BBa_M1115 Version 1 (Component)
Final lignin degradation construct

BBa_M1013
BBa_M1013 Version 1 (Component)
Complete Construct for Type I Phasin Secretion

BBa_M1014
BBa_M1014 Version 1 (Component)
Complete construct for phasin secretion together with zinc finger binding sites

BBa_M1030
BBa_M1030 Version 1 (Component)
Total construct Pha, Phb, Phc plus zinc fingers and promoter

BBa_M1031
BBa_M1031 Version 1 (Component)
Total construct Pha, Phb, Phc plus zinc fingers and promoter

BBa_K590062
BBa_K590062 Version 1 (Component)
Gli1-ADC HivC-AAR Construct

BBa_K611009
BBa_K611009 Version 1 (Component)
TetR Characterization Construct

BBa_K611010
BBa_K611010 Version 1 (Component)
LacI Repressor Characterization Construct

BBa_K611011
BBa_K611011 Version 1 (Component)
cI lambda Repressor Characterization Construct

BBa_K611012
BBa_K611012 Version 1 (Component)
pTet Characterization Construct

BBa_K611013
BBa_K611013 Version 1 (Component)
pLacI Characterization Construct

BBa_K611014
BBa_K611014 Version 1 (Component)
cI Repressible Promoter Characterization Construct

BBa_K535005
BBa_K535005 Version 1 (Component)
HydA1 - FeOx construction (Hydrogenase-ferredoxin fussion)

BBa_K615004
BBa_K615004 Version 1 (Component)
Zif268 binding site-His3-URA3 construct for one-hybrid selection

BBa_K823015
BBa_K823015 Version 1 (Component)
Pxyl, a xylose inducible promoter + its regulator XylR

BBa_K733002
BBa_K733002 Version 1 (Component)
<i>xylR</i>+<i>PxylA</i>: A xylose inducible promoter with its transcriptional regulator.

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.

SBOLDesigner CAD Tool
SBOLDesigner Version 3.1 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_K113021
BBa_K113021 Version 1 (Component)
Total construct: T7 polymerase + GFP/RFP + repressors + overlapping T7 promoter

BBa_K090502
BBa_K090502 Version 1 (Component)
Gram-Positive Xylose-Inducible Promoter

BBa_K143077
BBa_K143077 Version 1 (Component)
P43-gsiB GFP expression construct

BBa_K143078
BBa_K143078 Version 1 (Component)
P43-spoVG GFP expression construct

BBa_K143079
BBa_K143079 Version 1 (Component)
Pveg-spoVG GFP expression construct

BBa_K143080
BBa_K143080 Version 1 (Component)
P43-gsiB RFP expression construct

BBa_K143081
BBa_K143081 Version 1 (Component)
P43-spoVG RFP expression construct

BBa_K143082
BBa_K143082 Version 1 (Component)
Pveg-spoVG RFP expression construct

BBa_K082000
BBa_K082000 Version 1 (Component)
reconstruct strong RBS

Regional
BBa_K106676 Version 1 (Component)
Regional silencing construct

BBa_K112410
BBa_K112410 Version 1 (Component)
cadC promoter and GFP reporter construct

BBa_K116646
BBa_K116646 Version 1 (Component)
Intermediate part to construct a synchronizer

BBa_K116648
BBa_K116648 Version 1 (Component)
Intermediate part to construct a synchronizer

BBa_K152010
BBa_K152010 Version 1 (Component)
TB1 RNAi construct

BBa_K152011
BBa_K152011 Version 1 (Component)
GFP RNAi construct

BBa_K112409
BBa_K112409 Version 1 (Component)
RecA reporter GFP construct

BBa_K112411
BBa_K112411 Version 1 (Component)
cadAB promoter GFP reporter construct